VitriMed

Med.techn.Abt.der Mobil-Tec Elektronik GmbH

 

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VitriSafe Users Guide

Description and use: Vitrification of oocytes and embryos

"VitriSafe" is a special sample carrier for the use as a part of a closed system for Vitrification of oocytes and embryos. It is designed to fit into a straw of an outer of 3mm which shall be welded properly on both ends to avoid contact of liquid N2 . (recommendable straws of this type are e.g. the CBS High Security Straws 010286 and 010287 which have as well the advantage of having a separate compartment for identification).

Preventing the risk of potential contamination with pollutions in LN2 the use of a closed system is called as well "aseptic vitrification".

WARNING:

Read the complete direction for the use before use.

These procedures should only be performed by persons having adequate

training and familiarity with these techniques, especially with the special

features of closed (aseptic) vitrification.

To be used by, or under the direction of qualified persons in line with local

guidelines governing IN VITRO FERTILISATION , if applicable

Discard if product or packaging is damaged.

Identification

The probe can be identified by using the identification system of the enclosing straw.

Storage in liquid nitrogen

All traditional storage systems for straws of a length of 133mm can be used.

Instructions for use

Cooling

1. Prepare the enclosing straw with its identification label (e.g. CBS HS Straw system 010286

or 010287) and seal it on its end with the identification (e.g. CBS ID rod 019021 ff).

2. Prepare the sample for vitrification by the use of special media (e.g. VitriFreeze-S by

FertiPro/B) and according to an adequate protocol for vitrification with a closed systems!

3. Using a micropipette, carefully deposit a small drop of sample into the gutter a few

millimeters from the end. Maximum of 2 oocytes or embryos.

4. Immediately place the VitriSafe with the gutter ahead into the open end of the straw

(the VitriSafe will enter completely by turning the outer straw in a vertical position).

5. While still holding the straw in place, seal the open end using a heat sealer (e.g..

CBS/SYMS ).

6. Quickly plunge the entire straw into liquid nitrogen vertically.

7 Gently stir the straw in liquid nitrogen for a few seconds so as to avoid formation of an

isolating air bubble layer around the straw.

8. Transfer the straw quickly to the LN2 storage system

Warming

Quick operation and very fast thawing is essential

1. Prepare the special thawing/dilution media for vitrification with a closed systems!

(e.g. VitriThaw-S by FertiPro/B)

2. Identify the correct straw in the storage system

3. Transfer the straw quickly from the storage container to a small dewar filled with liquid

nitrogen reversely (vertically, with the labeling .end at the bottom and the part containing the

VitriSafe on top).

4. Lift the straw enough to expose the grooved handling rod. Make sure the end with the

sample remains immersed in the liquid nitrogen.

5. Cut the straw with either scissors or better with special stripping pliers*) just below the

handling rod, holding the straw with an adequate forceps*).

6. While still holding the straw with the forceps take care that the part of the straw where the

VitriSafe is with the gutter and the sample is still immersed in the liquid nitrogen (any

contact of the cutting edge or the VitriSafe with LN2 has to be avoided).

7. Then pull the VitriSafe with the sample quickly out of the straw and submerge the tip into

the 1st medium..

8. Proceed the thawing by the use of special media and according to an adequate protocol

for fast thawing of samples vitrified with a closed system! **)

Shelf-life

Three years from date of manufacture.

Sterilization: by Gamma-Radiation 25kGy

Reference Description Packaging

VIT-S-60 12 bags with 5 pieces each single sterile packed 60 pieces

*) Recommended and available special accessory:

"Stripping"-pliers, Handling-forceps und Holding-forceps

**) "Aseptic vitrification of blastocysts from infertile patients, egg donors and after IVM",

Vanderzwalmen et al. RBMonline 2009 (in publication)

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